Professor Emeritus of Biology at Brandeis University
Professor Emeritus of Biology at Brandeis University
CNRS Research Director
Combining normal mode analysis, image analysis, and deep learning for in vitro and in situ studies of continuous conformational variability of macromolecular complexes
Date and time: March 24th, 2021
Wednesday, at 8am PT / 11am ET / 4pm GMT / 5pm CET / Feb 18th midnight China
For the zoom and gather.town links, please join the One World Cryo-EM mailing list.
Note that we no longer use the zoom registration system, so old zoom links may not work. The new links will be sent via the mailing list.
HEMNMA is a method to analyze continuous conformational variability of purified macromolecular complexes (in vitro), introduced in 2014 (Jin et al., Structure 22:496-506, 2014). Its software with user friendly graphical interface is part of the open-source ContinuousFlex plugin of Scipion 2 and Scipion 3 (Harastani et al., Protein Science 29:223–236, 2020). HEMNMA combines single particle image analysis, normal mode analysis, and dimension reduction techniques to visualize the full distribution of states (conformational landscape) in a low-dimensional space (usually 2D or 3D space), from which one can obtain 3D reconstructions and movies of molecular motions along desired directions. After a brief reminder on HEMNMA, I will present current developments, including combining HEMNMA with deep learning to accelerate the conformational landscape determination and an extension of HEMNMA to analyze continuous conformational variability of macromolecular complexes in cells from in situ cryo electron tomography data.
One World Cryo-EM Pilot Poster Session
Time and date: 3/10/2021
March 10 11am-1pm EST,
March 10 8-10pm EST,
March 11 8am-10am EST
Location: gather.town (link available through our mailing list)
We are delighted to announce that we will be hosting a pilot virtual One World Cryo-EM Poster Session on March 10-11, 2021. This poster session, held on gather.town, will be a chance to discuss research with colleagues, share your own work, and socialize with others in the community.
A list of posters, presenters, and their availability can be found below or at the following link.
We have 3 live poster sessions on: March 10 11am-1pm EST, March 10 8-10pm EST, March 11 8am-10am EST.
If you are interested in presenting, please submit your poster on this application form. With your poster, you may optionally submit a 3-5 minute video to be displayed alongside your poster for visitors outside of live poster sessions. More information and details are provided in the form.
Please make sure that you are on our mailing list. This is the only place where the links to the gather.town poster session will be posted. As always, you can find more information at https://cryoem.world.
We are excited to host this poster session, and hope that you will join us.
As a participant, you agree that any information presented in the One World Cryo-EM poster session, whether in a poster, talk, video, or discussion, is private communication and that the information is not for public use.
You agree not to record the information by any means and not to quote or publish it without the written permission of the presenter. This includes posting information on social media, in publications, and on blogs.
You understand that the posters are not peer-reviewed and not archivable.
You understand that it is up to each participant to follow these restrictions. The organizers are not responsible for enforcing these restrictions.
Note that the list of poster titles and authors will be available on the OWCryo-EM website. Therefore, you may tweet a recommendation to visit poster #123 titled x by y (#OWCryoEMPosters) without referencing the content.
As a presenter, you may post your own poster on Twitter and invite people to retweet it and meet you at your poster #OWCryoEMPosters.
Q: What is gather.town? What do posters there look like?
A: This is what a poster looks like on gather.town: https://gather.town/app/5Qb5FSmPM0wtDvpd/sample_poster (the actual session is expected to have a slightly larger number of posters).
Q: How do I view the gather.town session?
A: Please make sure that you are on our mailing list. This is the only place where the links to the gather.town poster session will be posted.
Q: What is the poster selection process?
A: In this pilot poster session, we expect to accept all novel work that satisfies the topic, quality, and form requirements.
Q: How do I submit a poster?
A: Please submit your completed poster on this application form. You are not required to submit a title and abstract before submitting the full poster.
Q: What are the requirements for the poster?
A: Posters should be in an image format (eg .png, .jpg, but not .pdf), and should be at least 4000×6000 pixels. You may resubmit posters up to 10 times until February 22nd. These will be collectively posted in gather.town, and we will let you know your poster ID number. Here is a poster size example.
Q: What are the requirements for the video?
A: Videos are optional, and should be between 3-5 minutes long and provide viewers a brief description of your poster. These will be posted next to your poster for visitors that cannot make the live session, or to provide visitors context about your poster.