The One World Cryo-EM seminar series is a platform for discussion of algorithms, computational methods and mathematical problems in cryo-EM.
 

Online, Every Other Wednesday, at 8am PT / 11am ET/ 4pm BST / 5pm CET / 11pm China.

Registration has changed. For zoom links and announcements, please register to our mailing list. This is a read-only mailing list (users cannot post). Please use your institution/ company email – we approve registrations manually and this helps us know who you are . You may receive an email asking you to confirm your registration, it is important that you click the link, otherwise we will not see your request.

Organizers: Joakim Andén, Dorit Hanein, Roy R. Lederman and Steven J. Ludtke


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Next Talk


Amit Singer

Amit Singer

Professor of Mathematics at Princeton University

Wilson Statistics: Derivation, Generalization, and Applications to Cryo-EM

Date and time: June 16, 2021

Wednesday, at 8am PT / 11am ET / 3pm GMT / 5pm CET / 11pm China

For the zoom and gather.town links, please join the One World Cryo-EM mailing list.
Note that we no longer use the zoom registration system, so old zoom links may not work. The new links will be sent via the mailing list.

The power spectrum of proteins at high frequencies is remarkably well described by the flat Wilson statistics. Wilson statistics therefore plays a significant role in X-ray crystallography and more recently in cryo-EM. Specifically, modern computational methods for three-dimensional map sharpening and atomic modelling of macromolecules by single particle cryo-EM are based on Wilson statistics. In this talk we provide the first rigorous mathematical derivation of Wilson statistics. The derivation pinpoints the regime of validity of Wilson statistics in terms of the size of the macromolecule. Moreover, the analysis naturally leads to generalizations of the statistics to covariance and higher order spectra. These in turn provide theoretical foundation for assumptions underlying the widespread Bayesian inference framework for three-dimensional refinement and for explaining the limitations of autocorrelation based methods in cryo-EM.

Last Talk


Lauren Ann Metskas

Lauren Ann Metskas

Postdoctoral Fellow at California Institute of Technology
Incoming Assistant Professor at Purdue University

Illuminating the Map: Current Practices, Challenges, and Future Applications of Cryo-CLEM

Date and time: May 19, 2021

Wednesday, at 8am PT / 11am ET / 3pm GMT / 5pm CET / 11pm China

For the zoom and gather.town links, please join the One World Cryo-EM mailing list.
Note that we no longer use the zoom registration system, so old zoom links may not work. The new links will be sent via the mailing list.

Correlated cryo-light and cryo-electron microscopy (cryo-CLEM) has become an increasingly popular method for combining the resolving power of cryo-EM with the specificity of fluorescence. Although cryo-fluorescence microscopy suffers from optical limitations, it is a powerful way to target the resolving power of cryo-EM toward proteins of interest in heterogeneous cellular environments. Two large areas of methods developments are ongoing in both hardware and software: improving the correlation precision and accuracy to facilitate single-protein targeting, and expanding the cryo-fluorescence toolkit to include room-temperature approaches capable of targeting functions or specific protein conformations. This webinar will begin with a theoretical overview of current obstacles and considerations for software developers seeking to improve correlation precision and accuracy, and conclude with our recent efforts to improve cryo-fluorescence data quality, including characterizing the effect of cryo temperatures on fluorophores and establishing function-based localization in cryo-CLEM.

Other “One Worlds” (not affiliated): https://www.owprobability.org/other-worlds