The One World Cryo-EM seminar series is a platform for discussion of algorithms, computational methods and mathematical problems in cryo-EM.
 

Online, Every Other Wednesday, at 8am PT / 11am ET/ 4pm GMT / 5pm CET / 11am Thursday China.

Registration has changed. For zoom links and announcements, please register to our mailing list. This is a read-only mailing list (users cannot post). Please use your institution/ company email – we approve registrations manually and this helps us know who you are . You may receive an email asking you to confirm your registration, it is important that you click the link, otherwise we will not see your request.

Organizers: Joakim Andén, Dorit Hanein, Roy R. Lederman and Steven J. Ludtke


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Next Talk

David DeRosier

David DeRosier

Professor Emeritus of Biology at Brandeis University

Where in the cell is my protein: revisited.

Date and time: April 7, 2021

Wednesday, at 8am PT / 11am ET / 3pm GMT / 5pm CET / 11pm China

For the zoom and gather.town links, please join the One World Cryo-EM mailing list.
Note that we no longer use the zoom registration system, so old zoom links may not work. The new links will be sent via the mailing list.

Cryo electron tomograms (cryo-ET) are nothing short of amazing. There is so much to see, but wouldn’t it be nice if we could identify all those structural features in terms of their molecular identity? The combination of cryo-single molecule localization microscopy (cryo-SMLM) and cryo-ET should provide a pathway to making such identification. What are the challenges to realizing the combination’s full potential? Why the fish tank?


Last Talk

Slavica Jonic

Slavica Jonic

CNRS Research Director

Combining normal mode analysis, image analysis, and deep learning for in vitro and in situ studies of continuous conformational variability of macromolecular complexes

Date and time: March 24th, 2021
Wednesday, at 8am PT / 11am ET / 3pm GMT / 4pm CET / 11pm China

For the zoom and gather.town links, please join the One World Cryo-EM mailing list.
Note that we no longer use the zoom registration system, so old zoom links may not work. The new links will be sent via the mailing list.

HEMNMA is a method to analyze continuous conformational variability of purified macromolecular complexes (in vitro), introduced in 2014 (Jin et al., Structure 22:496-506, 2014). Its software with user friendly graphical interface is part of the open-source ContinuousFlex plugin of Scipion 2 and Scipion 3 (Harastani et al., Protein Science 29:223–236, 2020). HEMNMA combines single particle image analysis, normal mode analysis, and dimension reduction techniques to visualize the full distribution of states (conformational landscape) in a low-dimensional space (usually 2D or 3D space), from which one can obtain 3D reconstructions and movies of molecular motions along desired directions. After a brief reminder on HEMNMA, I will present current developments, including combining HEMNMA with deep learning to accelerate the conformational landscape determination and an extension of HEMNMA to analyze continuous conformational variability of macromolecular complexes in cells from in situ cryo electron tomography data.     


Other “One Worlds” (not affiliated): https://www.owprobability.org/other-worlds