2021/05/19 Lauren Ann Metskas

Lauren Ann Metskas

Lauren Ann Metskas

Postdoctoral Fellow at California Institute of Technology
Incoming Assistant Professor at Purdue University

Illuminating the Map: Current Practices, Challenges, and Future Applications of Cryo-CLEM

Date and time: May 19, 2021

Wednesday, at 8am PT / 11am ET / 3pm GMT / 5pm CET / 11pm China

For the zoom and gather.town links, please join the One World Cryo-EM mailing list.
Note that we no longer use the zoom registration system, so old zoom links may not work. The new links will be sent via the mailing list.

Correlated cryo-light and cryo-electron microscopy (cryo-CLEM) has become an increasingly popular method for combining the resolving power of cryo-EM with the specificity of fluorescence. Although cryo-fluorescence microscopy suffers from optical limitations, it is a powerful way to target the resolving power of cryo-EM toward proteins of interest in heterogeneous cellular environments. Two large areas of methods developments are ongoing in both hardware and software: improving the correlation precision and accuracy to facilitate single-protein targeting, and expanding the cryo-fluorescence toolkit to include room-temperature approaches capable of targeting functions or specific protein conformations. This webinar will begin with a theoretical overview of current obstacles and considerations for software developers seeking to improve correlation precision and accuracy, and conclude with our recent efforts to improve cryo-fluorescence data quality, including characterizing the effect of cryo temperatures on fluorophores and establishing function-based localization in cryo-CLEM.