Carlos Oscar Sorzano
Staff Researcher at the National Center of Biotechnology (CSIC)
Date and time: January 18th, 2023
Wednesday, at 8am PST / 11am EST / 4pm GMT / 5pm CET / midnight China
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Bias, variance and map validation in Single Particle Analysis by CryoEM
Single Particle Analysis by CryoEM has established as a mature technique to elucidate the three-dimensional structure of biological macromolecules. Once the experimental data is acquired, reconstructing the macromolecule’s map involves the estimation of millions of parameters. Due to the low Signal-to-Noise Ratio, this estimation is prone to mistakes. Depending on the error size, these mistakes may result into small perturbations in the reconstructed map (variance) or large artifacts (bias). In this talk we will discuss different kinds of mistakes that can be committed and how to identify and tackle them.